本实验采用改进的方法对油菜花粉的蛋白进行提取，利用SDS-PAGE和Westernblotting的方法对油菜花粉提取液进行分离和免疫学鉴定，进一步通过离子交换对主要过敏原进行纯化，也发现油菜花粉主要过敏原为10000、15000的蛋白，其中15000过敏原是否为profilin还需进一步进行鉴定。

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